Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Functional analysis of two maize cDNAs encoding T7-like RNA polymerases.

C C Chang1, J Sheen, M Bligny

  • 1Boyce Thompson Institute for Plant Research, Tower Road, Cornell University, Ithaca, New York 14853-1801, USA.

The Plant Cell
|May 20, 1999
PubMed
Summary

Two maize genes, rpoTm and rpoTp, encode organelle RNA polymerases. rpoTm is linked to mitochondrial transcription, while rpoTp is a plastid component, with expression varying by tissue and development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

cDNA sequence analysis and expression of the a chain of beta-bungarotoxin from Bungarus multicinctus (Taiwan banded krait).

Biochemical and biophysical research communications·1996
Same author

A comparison of the propensity for gene amplification between near-tetraploid and near-diploid V79 clones resistant to 150 nM methotrexate.

Carcinogenesis·1996
Same author

Chromosomal translocations cause deregulated BCL6 expression by promoter substitution in B cell lymphoma.

The EMBO journal·1995
Same author

Regulation and immunolocalization of acyl-coenzyme A: cholesterol acyltransferase in mammalian cells as studied with specific antibodies.

The Journal of biological chemistry·1995
Same author

Hydration, not silicone, modulates the effects of keratinocytes on fibroblasts.

The Journal of surgical research·1995
Same author

cDNA sequence analysis and expression of alpha-bungarotoxin from Taiwan banded krait (Bungarus multicinctus).

Biochemical and biophysical research communications·1995

Area of Science:

  • Plant molecular biology
  • Organelle gene expression
  • Maize genetics

Background:

  • T7-like RNA polymerases are crucial for gene transcription in various organisms.
  • Understanding organelle transcription machinery in plants is essential for cellular processes.

Purpose of the Study:

  • To characterize two maize cDNAs, rpoTm and rpoTp, encoding putative T7-like RNA polymerases.
  • To determine the cellular localization and function of the proteins encoded by rpoTm and rpoTp.
  • To investigate the expression patterns of rpoTm and rpoTp in different maize tissues and conditions.

Main Methods:

  • In vivo cellular localization using green fluorescent protein (GFP) fusion proteins.
  • Antibody-based detection and activity assays for mitochondrial polypeptides.

Related Experiment Videos

  • Immunoblot analysis and antibody-linked polymerase assays for plastid RNA polymerase.
  • Quantitative reverse transcription-polymerase chain reaction (RT-PCR) for gene expression analysis.

    • Main Results:

    • rpoTm and rpoTp encode proteins localized to mitochondria and plastids, respectively.
    • The rpoTm product is involved in maize mitochondrial transcription, confirmed by antibody inhibition.
    • The rpoTp product is identified as a component of the plastid RNA polymerase.
    • Both genes are constitutively expressed, but rpoTm is more abundant in nonphotosynthetic tissues, and rpoTp increases with chloroplast development.

    Conclusions:

    • rpoTm and rpoTp encode constitutive components of maize mitochondrial and plastid transcription machinery.
    • Expression of these organelle RNA polymerases is modulated during plant development and in response to environmental factors.