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Updated: Aug 11, 2026

09:49
Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability
Published on: April 2, 2015
[Polydispersity of Serratia marcescens nuclease at optimal pH values]
M N Filimonova1, M J Benedik, N G Urazov
1Kazan State University, Russia.
Prikladnaia Biokhimiia I Mikrobiologiia
|May 20, 1999
Abstract:
Treatment with dimethyl suberimidate, a cross-linking bifunctional agent, showed that Sm1 and Sm2 nucleases of Serratia marcescens B10M1 are polydisperse in solution and consist of monomers and dimers at the level of pH optimal for the enzyme activity. The data suggest that nucleases from the strain B10M1 and any other strain are polydisperse at pH optimum if their amino acid sequences are identical.
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