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Related Experiment Videos

Estimating population size by genotyping faeces.

M H Kohn1, E C York, D A Kamradt

  • 1Department of Biology, University of California, Los Angeles 90095-1606, USA. michaelk@biology.ucla.edu

Proceedings. Biological Sciences
|May 20, 1999
PubMed
Summary
This summary is machine-generated.

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Estimating wildlife populations is challenging. This study presents a non-invasive method using coyote (Canis latrans) DNA from scat to determine population size, sex ratio, and even family relationships.

Area of Science:

  • Ecology
  • Conservation Biology
  • Wildlife Management

Background:

  • Accurate population size estimation is crucial for wildlife management and conservation.
  • Non-invasive genetic sampling offers a promising alternative to traditional methods for studying elusive species.

Purpose of the Study:

  • To develop and validate a general, non-invasive method for estimating large mammal population size using fecal DNA.
  • To apply this method to a coyote (Canis latrans) population in the Santa Monica Mountains, California.

Main Methods:

  • Systematic collection of presumed coyote feces along established routes.
  • DNA extraction and species/sex identification using mitochondrial and Y-chromosome DNA.
  • Microsatellite genotyping of fecal DNA.

Related Experiment Videos

  • Population size estimation using rarefaction analysis of genotypes.

    • Main Results:

    • The method successfully provided a point count estimate of the coyote population size and sex ratio.
    • Analysis of fecal genotypes allowed for inference of home range use, paternity, and kinship.

    Conclusions:

    • Non-invasive fecal genotyping is a versatile and effective tool for wildlife population assessment.
    • This approach can provide valuable insights into social structure and individual ecology beyond simple population counts.