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Updated: Feb 10, 2026
CIRCLE-Seq for Interrogation of Off-Target Gene Editing
Published on: November 1, 2024
E2F target genes and cell-cycle checkpoint control
1Centro di Genetica Evoluzionistica C.N.R., c/o Universita La Sapienza, Rome, Italy.
Abstract:
In this review, we will focus on the role played by transcription factors of the E2F/DP family in controlling the expression of genes that carry out important cell-cycle control functions, thereby ensuring ordered progression through the mammalian cell division cycle. The emerging picture is that cell-cycle progression depends on the execution of a regulatory cascade of gene expression, driven by E2F/DP transcription factors, which are in turn regulated by the products of some of these genes. That E2F factors are potent regulators of cell-cycle checkpoints in mammalian cells is supported by experiments demonstrating that ectopic expression of individual E2F family members is sufficient to modulate cell proliferation and apoptosis. It is also clear that deregulation of E2F activity will result in the loss of particular checkpoint controls, thereby predisposing cells to malignant conversion.
Insights
Transcription factors of the E2F/DP family regulate gene expression critical for mammalian cell division. Dysregulation of E2F activity can lead to uncontrolled cell proliferation and cancer.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- The cell division cycle requires precise control of gene expression.
- Transcription factors play a crucial role in regulating gene expression.
- The E2F/DP family of transcription factors is implicated in cell cycle control.
Purpose of the Study:
- To review the role of E2F/DP transcription factors in regulating cell-cycle control genes.
- To elucidate the regulatory cascade driven by E2F/DP factors in mammalian cell division.
- To discuss the consequences of E2F deregulation on cell cycle checkpoints and malignant transformation.
Main Methods:
- Literature review of studies on E2F/DP transcription factors.
- Analysis of experimental evidence on gene expression regulation during the cell cycle.
- Examination of the impact of E2F modulation on cell proliferation and apoptosis.
Main Results:
- E2F/DP transcription factors drive a cascade of gene expression essential for ordered cell cycle progression.
- E2F/DP activity is regulated by the products of the genes they control, forming a feedback loop.
- Ectopic expression of E2F family members demonstrates their potent regulatory role in cell proliferation and apoptosis.
- Deregulation of E2F activity disrupts cell cycle checkpoints, contributing to cellular transformation.
Conclusions:
- E2F/DP transcription factors are central regulators of the mammalian cell division cycle.
- The precise control of E2F/DP activity is vital for maintaining genomic stability.
- Disruption of E2F-mediated gene expression control is a key event in cancer development.
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