Transcriptional repression by the zinc finger protein REST is mediated by titratable nuclear factors

The zinc finger protein REST (RE-1 silencing transcription factor) is a transcriptional repressor that inhibits neuronal gene transcription in non-neuronal tissues. REST may represent a master regulator of neuronal gene expression. REST contains two repressor domains located at the N- and C-termini of the molecule. To investigate the molecular mechanism of transcriptional repression by REST, in vivo competition experiments were performed. Both repression domains were expressed in the nucleus as fusion proteins with S. japonicum glutathione S-transferase (GST). The ability of these fusion proteins to block transcriptional repression mediated by the repressor domains of REST was tested. The results show that transcriptional repression by the N-terminal repression domain of REST could be overcome by expression of a GST fusion protein encoding the N-terminal, but not C-terminal repression domain, and vice versa, suggesting that both repression domains have to interact with distinct nuclear factors to exhibit biological activity. The GST-REST fusion proteins had no effect upon transcriptional repression mediated by the KRAB (Krüppel-associated box) domain, a strong mammalian repressor domain, or the repressor domain derived from the thyroid hormone receptors alpha. We conclude that REST has to interact with at least two distinct nuclear factors to inhibit transcription. These factors are distinct from the mammalian corepressor proteins KAP-1/KRIP-1 and N-CoR that mediate repression by the KRAB domain or the thyroid hormone receptor alpha. Thus, mammalian transcriptional repressors utilize different mechanisms to inhibit transcription by using different kinds of protein-protein interactions.