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Related Experiment Videos

Evolutionary DNA variation in the genus hypochaeris

Cerbah1, Coulaud, Brown

  • 1Laboratoire d'Evolution et Systematique, U.R.A. C.N.R.S. 2154, Universite Paris XI, F-91405 Orsay cedex.

Heredity
|May 21, 1999
PubMed
Summary

Genome size in Hypochaeris species varies significantly, with interspersed repeated sequences likely driving these changes. DNA content evolution involved both reduction and expansion across lineages.

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Area of Science:

  • Plant genomics
  • Evolutionary biology
  • Cytogenetics

Background:

  • The genus Hypochaeris exhibits considerable morphological diversity.
  • Understanding nuclear DNA variation is crucial for plant systematics and evolution.

Purpose of the Study:

  • To quantify genome size and GC content in eight Hypochaeris species.
  • To investigate patterns of nuclear DNA variation within the genus.
  • To identify factors contributing to genome size variation.

Main Methods:

  • Flow cytometry was used to determine genome size (2C DNA content) and base composition (GC%).
  • Phylogenetic analysis was employed to infer evolutionary relationships.

Main Results:

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  • A nearly fivefold range of genome size variation was observed, from 1.68 pg in H. cretensis to 8.10 pg in H. uniflora.
  • DNA content variation was greater between taxonomic sections than within sections.
  • No correlation was found between 2C DNA content and GC%, suggesting GC or AT fractions are not primary drivers of genome size variation.
  • Interspersed repeated sequences are implicated as the main source of genome size variation, given the low amount of heterochromatin.

    • Conclusions:

    • Genome size evolution in Hypochaeris is characterized by DNA loss in some lineages and DNA gain in others.
    • Interspersed repeated DNA sequences play a significant role in shaping genome size diversity within the genus.