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Circular dichroism analysis of insect cell expressed herpes simplex virus type I single-stranded DNA-binding protein
1Biomedizinische Forschungsgesellschaft mbH, c/o Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität Wien, Veterinaerplatz 1, Vienna, A-1210, Austria. Martin.Spatz@vu-wien.ac.at
Protein Expression and Purification
|May 25, 1999
Abstract:
We have developed a baculovirus expression system for the rapid and efficient production of large quantities (>5 mg/10(8) cells) of ICP8. The recombinant ICP8 is fully functional and binds to single-stranded DNA. Secondary structure calculations from circular dichroism measurements indicate a content of 34.5% alpha-helix and 15.4% beta-sheet. This is the first structural report for ICP8 using CD analysis, which will be very useful for high-throughput assay development and mechanistic studies.