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Updated: Aug 12, 2026
Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry
Published on: May 21, 2018
Expression of a lipocalin in prokaryote and eukaryote cells: quantification and structural characterization of
J M Chatel1, K Adel-Patient, C Créminon
1INRA-CEA, CEA, Laboratoire d'Etudes RadioImmunologique, DRM-SPI, Bat 136, CE Saclay, Gif Sur Yvette, 91191, France. chatel@dsvidf2@cea.fr
Abstract:
In this paper we quantify and characterize the expression of recombinant beta-lactoglobulin (rBLG) in prokaryote and eukaryote cells. In Escherichia coli we used the pET26 vector, which permits the secretion of rBLG in periplasm. We studied the expression of rBLG in COS-7 cells and in vivo in mouse tibialis muscle. The expression of rBLG was measured by two immunoassays specific, respectively, for BLG in its native and denatured conformation. We observed that rBLG was essentially expressed in a denatured form in E. coli even in the periplasm, whereas rBLG in eukaryote cells was found in its native conformation.
