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Related Experiment Videos

Increasing DNA transfer efficiency by temporary inactivation of host restriction.

R A Edwards1, R A Helm, S R Maloy

  • 1Department of Microbiology, University of Illinois, Urbana-Champaign 61801, USA.

Biotechniques
|May 25, 1999
PubMed
Summary

Researchers developed a simple method to temporarily disable bacterial host restriction systems. This technique significantly enhances the efficiency of foreign DNA transfer into common bacterial hosts like E. coli and Salmonella.

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Area of Science:

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  • Genetics