Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Two-color GFP expression system for C. elegans.

D M Miller1, N S Desai, D C Hardin

  • 1Vanderbilt University Medical Center, Nashville, TN, USA.

Biotechniques
|May 25, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of Heatwaves and Declining NO<sub>x</sub> on Nocturnal Monoterpene Oxidation in the Urban Southeastern United States.

Journal of geophysical research. Atmospheres : JGR·2024
Same author

Neighborhood disadvantage, race, and clinical outcomes in neuromyelitis optica spectrum disorder.

Multiple sclerosis (Houndmills, Basingstoke, England)·2024
Same author

Imaging Dendritic Spines in Caenorhabditis elegans.

Journal of visualized experiments : JoVE·2021
Same author

Field Evaluations of Sulfuryl Fluoride Fumigation for Control of the Common Bed Bug (Hemiptera: Cimicidae), Using a 1.9× Dosage Factor in Motor Vehicles and Filled Cargo Trailers.

Journal of economic entomology·2021
Same author

Cognitive processing speed in multiple sclerosis clinical practice: association with patient-reported outcomes, employment and magnetic resonance imaging metrics.

European journal of neurology·2020
Same author

Multiple Sclerosis Performance Test: validation of self-administered neuroperformance modules.

European journal of neurology·2020
Same journal

Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNΔ7 truncated protein.

BioTechniques·2026
Same journal

Antigen retrieval-immunofluorescence on free floating sections to visualize the liver lobule and its cellular makeup.

BioTechniques·2026
Same journal

Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line.

BioTechniques·2026
Same journal

Strand-specific quantification of L1 ORF0 and related transcripts by multiplex reverse transcription with tagged primers.

BioTechniques·2026
Same journal

Why and when should we choose digital PCR?

BioTechniques·2026
Same journal

Quantitative and unbiased lung alveolar septum assessment in an LPS experimental mouse model using 2D-spatial correlation image analysis from hematoxylin and eosin slides.

BioTechniques·2026
See all related articles

Researchers developed cyan and yellow fluorescent proteins (CFP and YFP) from green fluorescent protein (GFP) for dual-protein tracking in Caenorhabditis elegans. This enables simultaneous visualization of two distinct proteins within the organism.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Green fluorescent protein (GFP) from Aequora victoria is a vital tool in molecular biology.
  • Simultaneous tracking of multiple proteins is crucial for understanding complex biological processes.
  • Existing fluorescent proteins may have limitations in spectral overlap or brightness for dual-labeling studies.

Purpose of the Study:

  • To develop and characterize novel cyan (CFP) and yellow (YFP) fluorescent protein variants.
  • To enable simultaneous visualization and tracking of two distinct proteins in Caenorhabditis elegans.
  • To provide researchers with new tools for studying protein expression and localization.

Main Methods:

  • Engineering of modified Aequora victoria green fluorescent protein (GFP) variants (CFP and YFP) with specific amino acid substitutions.

Related Experiment Videos

  • Construction of expression vectors containing CFP and YFP coding regions within a high-activity C. elegans expression system.
  • Utilizing previously characterized promoters and localization signals for targeted protein expression in C. elegans.
  • Employing specialized filter sets to distinguish CFP and YFP fluorescence spectra for simultaneous visualization.

    • Main Results:

    • Successfully generated functional CFP and YFP derivatives with distinct spectral properties.
    • Demonstrated the co-expression and visualization of CFP and YFP in C. elegans neurons and muscle cells.
    • Confirmed the utility of CFP and YFP for simultaneous tracking of two different proteins in vivo.
    • Developed and made available a series of expression vectors for CFP and YFP.

    Conclusions:

    • Modified CFP and YFP provide a robust system for dual-labeling studies in C. elegans.
    • These novel fluorescent proteins enhance the ability to investigate protein dynamics and interactions.
    • The availability of these vectors will facilitate advanced research in developmental biology and neuroscience using C. elegans.