Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Single bilayer vesicles prepared without sonication. Physico-chemical properties.

J Brunner, P Skrabal, H Hauser

    Biochimica Et Biophysica Acta
    |December 2, 1976
    PubMed
    Summary

    Researchers created uniform, single-shelled lecithin vesicles without sonication. This method yields pure lecithin vesicles, offering an alternative to sonication for vesicle preparation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A soft matter computer for soft robots.

    Science robotics·2020
    Same author

    Representative Factor Generation for the Interactive Visual Analysis of High-Dimensional Data.

    IEEE transactions on visualization and computer graphics·2015
    Same author

    [Radiological support for the cancer clinic in Dar es Salaam, Tanzania: 30 years of Tanzania tumor aid Heidelberg].

    Der Radiologe·2015
    Same author

    Laparoscopic versus open appendectomy for complicated appendicitis in high risk patients.

    International journal of colorectal disease·2014
    Same author

    Exploiting short-term memory in soft body dynamics as a computational resource.

    Journal of the Royal Society, Interface·2014
    Same author

    Scientific storytelling using visualization.

    IEEE computer graphics and applications·2014

    Area of Science:

    • Biochemistry
    • Materials Science
    • Physical Chemistry

    Background:

    • Lecithin vesicles are crucial in drug delivery and biomaterials.
    • Traditional sonication methods can be energy-intensive and may damage vesicle integrity.
    • Developing alternative, non-sonication methods for vesicle preparation is of significant interest.

    Purpose of the Study:

    • To develop a novel method for preparing single-shelled lecithin vesicles without sonication.
    • To characterize the morphology and properties of these non-sonicated vesicles.
    • To compare these vesicles with those prepared by sonication.

    Main Methods:

    • Solubilizing unsonicated lecithin dispersions with sodium cholate.
    • Forming mixed lecithin-cholate micelles.

    Related Experiment Videos

  • Removing detergent via gel filtration on Sephadex G-50 to obtain pure vesicles.

    • Main Results:

    • Successfully prepared single-shelled lecithin vesicles of uniform size (300 Å diameter).
    • Obtained a homogeneous population of pure lecithin vesicles, free of multilamellar structures.
    • Vesicle diameter was larger than sonicated vesicles, with bilayer curvature allowing similar packing densities.

    Conclusions:

    • The sodium cholate and gel filtration method is effective for producing uniform, single-shelled lecithin vesicles without sonication.
    • These non-sonicated vesicles exhibit distinct properties compared to sonicated ones.
    • This method provides a valuable alternative for lecithin vesicle preparation.