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Related Experiment Videos

Progesterone receptor expression in the human placenta.

Y G Shanker1, A J Rao

  • 1Department of Biochemistry, Indian Institute of Science, Bangalore.

Molecular Human Reproduction
|May 25, 1999
PubMed
Summary

This study found progesterone receptors (PR) in the human placenta, with lower amounts during late pregnancy. Further analysis revealed only the A-form of PR is present in placental tissue.

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Area of Science:

  • Reproductive biology
  • Endocrinology
  • Molecular genetics

Background:

  • Progesterone receptors (PR) play crucial roles in reproductive processes.
  • The presence and specific forms of PR in the human placenta are not fully elucidated.
  • Understanding placental PR is vital for comprehending pregnancy physiology.

Purpose of the Study:

  • To confirm the presence of progesterone receptors (PR) in the human placenta.
  • To investigate the levels of PR during different stages of gestation.
  • To identify the specific isoforms of PR expressed in the human placenta.

Main Methods:

  • Reverse transcriptase-polymerase chain reaction (RT-PCR) to detect PR gene expression.
  • Electrophoretic mobility shift assays (EMSA) to analyze protein-DNA interactions.
  • Supershift analysis using isoform-specific antibodies to identify PR types.

Main Results:

  • Progesterone receptors (PR) were detected in the human placenta using RT-PCR.
  • PR levels were observed to be lower in late gestation compared to earlier stages.
  • EMSA revealed three specific DNA-binding complexes, all inhibited by unlabeled competitor DNA.
  • Supershift analysis confirmed the presence of only the A-form of progesterone receptor (PR-A) in placental nuclear extracts.

Conclusions:

  • The human placenta expresses progesterone receptors (PR).
  • PR levels decrease during late gestation.
  • The A-form of progesterone receptor (PR-A) is the predominant or sole isoform present in the human placenta.

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