Related Experiment Video

Updated: Oct 21, 2026

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

Published on: September 2, 2025

Cloning and molecular characterization of the citrate utilization citMCDEFGRP cluster of Leuconostoc

M Martín¹, M A Corrales, D de Mendoza

¹Centro de Investigaciones Biológicas (CSIC), Madrid, Spain.

FEMS Microbiology Letters

|May 26, 1999

Abstract:

The citMCDEFGRP cluster from Leuconostoc paramesenteroides involved in citrate utilization was cloned and its nucleotide sequence determined. Homology of the inferred gene products with characterized enzymes reveals that citP encodes the citrate permease P, citC the citrate ligase and citDEF the subunits of the citrate lyase of Leuconostoc. Moreover, it suggests that citM encodes a Leuconostoc malic enzyme. Analysis of citrate consumption by and citrate lyase activity of Lc. paramesenteroides J1[pCITJ1] showed that its citrate permease and its citrate lyase are induced by the presence of citrate in the growth medium. Southern blot analysis demonstrated that the citMCDEFGRP cluster is located in a plasmid.

More Related Videos

Generation of Marked and Markerless Mutants in Model Cyanobacterial Species

Generation of Marked and Markerless Mutants in Model Cyanobacterial Species

Published on: May 29, 2016

Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016

Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016

Published on: June 23, 2023

Related Concept Videos

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Articles linked to this work by shared authors, journal, and citation graph.

Importance of Neck Boundary Condition and Posture on Cervical Spine Response Assessed using a Detailed Finite Element Human Model in a Head-First Impact.

Annals of biomedical engineering·2025

Methodology to geometrically age human body models to average and subject-specific anthropometrics, demonstrated using a small stature female model assessed in a side impact.

Computer methods in biomechanics and biomedical engineering·2022

Awake prone positioning in nonintubated spontaneous breathing ICU patients with acute hypoxemic respiratory failure (PRONELIFE)-protocol for a randomized clinical trial.

Trials·2022

Sex, Age and Stature Affects Neck Biomechanical Responses in Frontal and Rear Impacts Assessed Using Finite Element Head and Neck Models.

Frontiers in bioengineering and biotechnology·2021

Importance of the cervical capsular joint cartilage geometry on head and facet joint kinematics assessed in a Finite element neck model.

Journal of biomechanics·2021

Validation of a Football Helmet Finite Element Model and Quantification of Impact Energy Distribution.

Annals of biomedical engineering·2019

See all related articles