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Isolation of developmentally regulated novel genes based on sequence identity and gene expression pattern
1Department of Life Science, College of Natural Sciences, Hanyang University, Seoul, Korea.
Molecules and Cells
|May 26, 1999
Summary
Researchers identified novel genes crucial for embryonic development and tissue differentiation using expressed sequence tags (ESTs) from embryonic stem (ES) cells. Many identified genes show dynamic expression patterns during differentiation and are also present in adult tissues.
Area of Science:
- Genetics
- Developmental Biology
- Molecular Biology
Background:
- Embryonic development and tissue differentiation involve complex gene regulation.
- Undifferentiated embryonic stem (ES) cells are a potential source for identifying novel genes involved in these processes.
Purpose of the Study:
- To identify novel genes expressed in ES cells that play roles in embryonic development and tissue differentiation.
- To analyze the expression patterns of these novel genes during ES cell differentiation and in adult tissues.
Main Methods:
- Utilized a modified expressed sequence tags (ESTs) approach to screen an ES cell cDNA library.
- Analyzed expression patterns using slot blot hybridization with developmental and tissue-specific probes.
- Assessed sequence homology against public databases.
Main Results:
- Identified several novel cDNA clones with significant developmental expression patterns.
- 27.7% of randomly chosen clones showed no homology to existing database sequences.
- Most novel clones exhibited differential regulation during ES cell differentiation, with some showing tissue-specific expression in adult tissues.
Conclusions:
- The employed strategy is efficient for novel gene discovery.
- The identified genes provide insights into gene networks governing embryonic development and differentiation.
- This approach is feasible for exploring genomewide gene expression in complex biological processes.