Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA extraction from activated sludges.

M Bourrain1, W Achouak, V Urbain

  • 1CEA/Cadarache DSV-DEVM, Laboratoire d'Ecologie Microbienne de la Rhizosphère (LEMiR), UMR163 CNRS-CEA, F-108 Saint-Paul-lez-Durance, France.

Current Microbiology
|May 26, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The association of dextran sodium sulfate to the bioactive agent I-modulia® attenuates Staphylococcus aureus virulence expression and δ-toxin production.

Microbial pathogenesis·2024
Same author

Genetic control of rhizosheath formation in pearl millet.

Scientific reports·2022
Same author

In-depth prospection of Avène Thermal Spring Water reveals an uncommon and stable microbial community.

Journal of the European Academy of Dermatology and Venereology : JEADV·2020
Same author

Annales de dermatologie et de venereologie·2017
Same author

[How I explore … a dento-maxillofacial dysmorphosis].

Revue medicale de Liege·2017
Same author

[ABOUT JUVENILE NASOPHARYNGEAL ANGIOFIBROMA].

Revue medicale de Liege·2015
Same journal

Promoter Engineering of the Surfactin Operon Enhances Surfactin Production in the Environmental Strain Bacillus subtilis RI4914.

Current microbiology·2026
Same journal

Staphylococcus aureus CC398 Lineage of the Human Clade Isolated from Bloodstream Infection and Colonization and Spread among Brazilian Patients Hospitalized during the COVID-19 Pandemic.

Current microbiology·2026
Same journal

Evaluation of the Immunogenicity of an Inactivated Vaccine against the Adenovirus Egg Drop Syndrome 1976 in Poultry.

Current microbiology·2026
Same journal

Sphingomonas chlorellae sp. nov., Isolated from Outdoor Photobioreactor Culturing Chlorella sp. HS2 with Soy Sauce Wastewater.

Current microbiology·2026
Same journal

Imported Cutaneous Leishmaniasis in India: Diagnosis and Treatment with Implications for Kala Azar Elimination.

Current microbiology·2026
Same journal

Integrated Screening and Antifungal Potential of Microalgae Extracts Against Botrytis californica.

Current microbiology·2026
See all related articles

Optimizing DNA extraction from activated sludge requires tailored cell lysis protocols. Stirring with cation exchange resin and lysozyme + SDS treatments yielded the highest DNA, enabling 16S rDNA analysis.

Area of Science:

  • Environmental microbiology
  • Molecular biology

Background:

  • Efficient DNA extraction from activated sludge is crucial for microbial community analysis.
  • Activated sludge samples present challenges due to complex floc structures and diverse microbial compositions.

Purpose of the Study:

  • To optimize cell lysis for DNA extraction from activated sludge.
  • To compare different floc dispersion and cell lysis methods for maximizing DNA yield.

Main Methods:

  • Tested two floc dispersion methods: sonication and stirring with cation exchange resin.
  • Evaluated three cell lysis treatments: lysozyme + SDS, sonication, and thermal shock.
  • Quantified DNA yield after applying lysis treatments in different sequences.

Main Results:

Related Experiment Videos

  • Stirring with cation exchange resin was more effective for floc dispersion than sonication.
  • Lysozyme + SDS was the most effective cell lysis treatment.
  • Optimal lysis sequences varied depending on sludge microbial composition and floc structure.
  • Achieved high DNA yields (approx. 50 mg DNA g-1 volatile suspended solids) suitable for PCR.

Conclusions:

  • Activated sludge DNA extraction protocols need to be adapted to specific sample characteristics.
  • The optimized methods provide high DNA yields and enable downstream molecular analyses like PCR amplification of 16S rDNA.