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Tilt angle dependence of cross-relaxation in off-resonance ROESY
B Cutting1, R Ghose, G Bodenhausen
1Section de Chime, Université de Lausanne, Lausanne, 1015, Switzerland.
Abstract:
We present an efficient experimental method to evaluate whether the effective cross-relaxation rate between a pair of spins vanishes when applying an off-resonance spin-lock field. It is shown that the cross-relaxation rate can be made to vanish even when the two spins concerned resonate at different offsets and experience significantly different tilt angles of their respective spin-lock fields. This is verified experimentally using a sample of 15N-labeled human ubiquitin, through selective excitation of chosen amide protons. The results are relevant for the quantitative interpretation of off-resonance ROESY experiments.
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