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High-cell-density cultivation of microorganisms.

D Riesenberg1, R Guthke

  • 1Hans Knöll Institute for Natural Product Research, Jena, Germany. driesenb@pmail.hki-jena.de

Applied Microbiology and Biotechnology
|May 26, 1999
PubMed
Summary

High-cell-density cultivation (HCDC) enhances microbial biomass and product yield. This review covers HCDC strategies, bioreactors, and advanced fermentation control for industrial applications.

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Area of Science:

  • Biotechnology
  • Microbial Cultivation

Background:

  • High-cell-density cultivation (HCDC) is crucial for maximizing microbial biomass and product formation in industrial biotechnology.
  • Achieving high cell densities presents challenges in maintaining optimal growth conditions and preventing product inhibition.

Purpose of the Study:

  • To provide a comprehensive overview of high-cell-density cultivation (HCDC) techniques for various microorganisms.
  • To discuss challenges, solutions, and advancements in HCDC, including bioreactor design and control strategies.

Main Methods:

  • Review of existing literature on HCDC strategies, bioreactor types, and fermentation control methods.
  • Analysis of strain improvements, feeding strategies, and advanced control systems like artificial neural networks and expert systems.

Main Results:

  • HCDC is applicable to bacteria, archaea, and eukarya (yeasts), with Escherichia coli serving as a model organism.
  • Various bioreactor designs (e.g., stirred-tank, membrane, gas-lift) and feeding strategies enable unlimited growth to very dense cultures.
  • Advanced fermentation control methods, including multivariate statistics and artificial intelligence, are essential for robust HCDC.

Conclusions:

  • Successful HCDC requires optimized strains, appropriate bioreactors, advanced feeding strategies, and sophisticated fermentation control.
  • These advancements are critical for meeting the growing industrial demand for microbial products through efficient cultivation.

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