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[Comparative data on the propagation of cells from commercial common and leukemia-free chick embryos]
Voprosy Virusologii
|July 1, 1976
Abstract:
Certain differences were found in comparative studies of optimal conditions for preparation and propagation of cell cultures from common and leukemia-free chick embryos. The yield of cells per 1 g of tissue was regularly higher by 12-20% with leukemia-free chick embryos than with common chick embryos. The viability of the latter under conditions of roller cultivation was lower. Monolayer cultures of cells from leukemia-free chick embryos formed 1 day earlier than those from cells of common chick embryos. The optimal medium for growth of leukemia-free chick embryo cells was 0.5% lactalbumin hydrolysate, and for common chick embryo cells--Eagle's medium.