Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characterisation of stem cell expression using two-dimensional electrophoresis.

A Pearce1, C N Svendsen

  • 1ESA Analytical, St. Ives, Cambs, United Kingdom. alisonp@genomicsoutions.co.uk

Electrophoresis
|May 27, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Socio-economic inequalities in uptake and timing of childhood vaccination: Taking a life course approach in an administrative cohort in Scotland.

Vaccine·2025
Same author

Silver trauma: a study of pre- and post-COVID-19 pandemic pan-CT scanning in elderly trauma patients in a large teaching hospital.

Clinical radiology·2024
Same author

Measurement of the Prompt D^{0} Nuclear Modification Factor in p-Pb Collisions at sqrt[s_{NN}]=8.16  TeV.

Physical review letters·2023
Same author

Measurement of the Branching Fractions B(B^{0}→pp[over ¯]pp[over ¯]) and B(B_{s}^{0}→pp[over ¯]pp[over ¯]).

Physical review letters·2023
Same author

Measurement of the Time-Integrated CP Asymmetry in D^{0}→K^{-}K^{+} Decays.

Physical review letters·2023
Same author

Observation of a Resonant Structure near the D_{s}^{+}D_{s}^{-} Threshold in the B^{+}→D_{s}^{+}D_{s}^{-}K^{+} Decay.

Physical review letters·2023

Human fetal brain stem cells were cultured using growth factors and differentiated with serum. Two-dimensional electrophoresis revealed significant protein expression changes within one hour of differentiation.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Stem Cell Research

Background:

  • Human fetal brain stem cells can be isolated and cultured.
  • Growth factors like epidermal growth factor (EGF) and fibroblast growth factor (FGF) promote stem cell proliferation.
  • Serum addition induces differentiation in these stem cells.

Purpose of the Study:

  • To investigate the protein expression changes during the early stages of human fetal brain stem cell differentiation.
  • To characterize the proteome of undifferentiated and differentiating human neural stem cells.

Main Methods:

  • Isolation and culture of human fetal brain stem cells.
  • Induction of proliferation using EGF and FGF.
  • Induction of differentiation using serum.

Related Experiment Videos

  • Protein analysis via two-dimensional (2-D) electrophoresis.

    • Main Results:

    • Stem cells formed spheres and maintained an undifferentiated state with mitogens.
    • Serum addition rapidly induced stem cell differentiation.
    • Two-dimensional electrophoresis showed marked differences in protein expression between undifferentiated and differentiated cells after 1 hour.

    Conclusions:

    • Human fetal brain stem cell differentiation involves rapid and significant alterations in protein expression.
    • 2-D electrophoresis is effective in detecting early proteomic changes during stem cell differentiation.
    • These findings provide insights into the molecular mechanisms underlying neural stem cell differentiation.