Tubulin and glial fibrillary acidic protein gene expression in developing fetal human brain at midgestation

Developmental alterations in the expression of glial fibrillary acidic protein (GFAP) and alpha-tubulin were examined at the level of mRNA and protein in human fetal brain between weeks 13-23 of gestation. Except for a transient increase at week 15, GFAP expression in the cytoskeletal (CSK) fraction was low until week 17, when it increased steadily to week 23, corresponding to the phase of glial proliferation. The developmental profile of alpha-tubulin in the CSK fraction displayed a biphasic pattern, with an initial rise between weeks 13-16 coinciding with the early phase of neuroblast multiplication, and a second rise between weeks 17-23 corresponding to the phase of glial proliferation. No significant difference in the spatial distribution of alpha-tubulin was found in different region of brain but GFAP expression varied with a higher level in cerebellum than that in cerebrum at late midgestation.