Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Efficient gene transfer by histidylated polylysine/pDNA complexes.

P Midoux1, M Monsigny

  • 1Centre de Biophysique Moléculaire, Glycobiologie CNRS UPR4301 and University of Orléans, rue Charles-Sadron, F-45071 Orléans Cedex 02, France. midoux@cnrs-orleans.fr

Bioconjugate Chemistry
|May 29, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preclinical evaluation of mRNA trimannosylated lipopolyplexes as therapeutic cancer vaccines targeting dendritic cells.

Journal of controlled release : official journal of the Controlled Release Society·2018
Same author

Effect of IL-1β, TNF-α and IGF-1 on trans-endothelial passage of synthetic vectors through an in vitro vascular endothelial barrier of striated muscle.

Gene therapy·2017
Same author

Evidence for plasmid DNA exchange after polyplex mixing.

Soft matter·2016
Same author

NF-kB related transgene expression in mouse tibial cranial muscle after pDNA injection followed or not by electrotransfer.

Biochimica et biophysica acta·2014
Same author

mRNA-based cancer vaccine: prevention of B16 melanoma progression and metastasis by systemic injection of MART1 mRNA histidylated lipopolyplexes.

Cancer gene therapy·2007
Same author

Non-viral vectors in cystic fibrosis gene therapy: progress and challenges.

Trends in biotechnology·2004

A novel cationic polymer enhances gene delivery by facilitating plasmid DNA escape from cellular vesicles. This breakthrough in non-viral transfection offers improved efficiency without harsh additives.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Background:

  • Plasmid/polylysine complexes improve DNA uptake but trap DNA in vesicles, hindering nuclear access.
  • Membrane-disrupting agents like chloroquine enhance transfection by aiding endosomal escape.

Purpose of the Study:

  • To develop a novel cationic polymer for efficient plasmid DNA (pDNA) transfection of mammalian cells.
  • To investigate the mechanism of endosomal escape mediated by the novel polymer.

Main Methods:

  • Synthesis of a polylysine polymer substituted with histidyl residues.
  • Complexation of the polymer with plasmid DNA (pDNA).
  • Transfection of various cell lines and assessment of efficiency, including in the presence of serum and bafilomycin A1.

Related Experiment Videos

Main Results:

  • The designed poly(histidyl)lysine polymer efficiently mediated pDNA transfection in multiple cell lines without chloroquine or fusogenic peptides.
  • Optimal transfection occurred with 38% +/- 5% histidyl substitution.
  • Transfection efficiency was robust in the presence of serum and significantly inhibited by bafilomycin A1, suggesting an endosomal mechanism.

Conclusions:

  • The poly(histidyl)lysine polymer represents a promising non-viral vector for gene delivery.
  • The polymer's efficacy is linked to pH-dependent protonation of imidazole groups within endosomes, facilitating pDNA release into the cytosol.