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Updated: Aug 8, 2026
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Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining
Published on: December 23, 2016
Respiratory syncytial virus infects the Bonnet monkey, Macaca radiata
E A Simoes1, A R Hayward, E M Ponnuraj
1Department of Pediatrics, Division of Pediatric Infectious Diseases, University of Colorado Health Sciences Center, Denver, CO 80262, USA.
Summary
The Bonnet monkey model offers a predictable way to study respiratory syncytial virus (RSV) in nonhuman primates. This model shows consistent changes, aiding research into RSV disease and potential vaccines.
Area of Science:
- Veterinary Virology
- Primate Models
- Respiratory Infectious Diseases
Background:
- Respiratory Syncytial Virus (RSV) causes significant respiratory illness in humans, necessitating robust animal models for research.
- Nonhuman primate models are valuable for studying RSV pathogenesis and evaluating interventions due to physiological similarities to humans.
Purpose of the Study:
- To establish and characterize a Bonnet monkey (Macaca radiata) model for respiratory syncytial virus (RSV) infection.
- To document the temporal sequence of virologic, cytologic, serologic, and inflammatory changes following experimental RSV infection in Bonnet monkeys.
Main Methods:
- Young feral Bonnet monkeys were infected endotracheally with a standardized dose of the Long strain of RSV.
- Samples were collected at various time points post-infection for viral recovery, antigen detection, mRNA analysis, histology, bronchoalveolar lavage (BAL) cell counts, and serology.
Main Results:
- RSV was consistently recovered from lungs, with peak viral titers on day 3 post-infection.
- RSV antigen and F protein mRNA were detected in lung tissues and immune cells from days 3-5.
- Progressive inflammatory changes, including bronchiolitis and alveolitis, peaked at day 7, with distinct cellular infiltrates in BAL fluid over time.
- Humoral immune responses, including neutralizing antibodies and IgG, were detected from day 5 onwards.
Conclusions:
- The Bonnet monkey model provides a reproducible system for studying RSV infection, mirroring key aspects of human disease.
- This model demonstrates a predictable timeline of virologic, cellular, and immune responses, making it suitable for evaluating RSV pathogenesis and vaccine efficacy.
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