Related Experiment Video
Updated: Oct 20, 2026

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
Abundance and distribution of bacteria carrying sltII gene in natural river water
1Genome Information Research Center, Graduate School of Pharmacy, Osaka University, Japan.
Abstract:
Direct in situ PCR with HNPP/Fast Red TR was used to enumerate bacteria carrying the sltII gene in river water. By direct in situ PCR with a sltII-specific EVS primer, 10(2)-10(5) cells ml-1 of bacteria carrying the sltII gene were detected from all sampling sites, except the site nearest to the source of the river, while 10(2)-10(4) cells ml-1 of Escherichia coli O157:H7 were detected using a direct fluorescent antibody staining method. These results indicate that such bacteria are commonly distributed in natural river water. Direct in situ PCR with HNPP/Fast Red TR is a useful tool for detecting cells carrying specific genes, such as verotoxin-producing bacteria in natural environments.
Related Concept Videos
Gene Regulation in Microbial Communities: Quorum Sensing
Bacterial Signaling
Reservoir of Infection
Freshwater Microbial Ecology
Antibiotic Selection
Conjugation

