High-performance liquid chromatographic determination of 1,3-diethyl-2-thiobarbituric acid--malonaldehyde adduct in fish meat
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Updated: Oct 20, 2026
Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Published on: May 12, 2020
T Sakai1, A Habiro, S Kawahara
1Faculty of Agriculture, Miyazaki University, Japan. a0b102u@cc.miyazaki-u.ac.jp
Abstract:
The reaction conditions of 1,3-diethyl-2-thiobarbituric acid (DETBA)-malonaldehyde (MA) adduct formation were examined in order to analyze MA in fish tissue by high-performance liquid chromatography. A reaction mixture containing 4 mM butyl hydroxytoluene was heated at 100 degrees C for 150 min and the DETBA-MA adduct formed was separated by a Inertsil ODS column for 20 min. The detection limit was 5 pmol.
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