Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell cycle dependent subcellular distribution of Cdc25B subtypes.

E S Woo1, R L Rice, J S Lazo

  • 1Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh, Pennsylvania 15261, USA.

Oncogene
|May 29, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metastasis-associated phosphatase PRL-2 regulates tumor cell migration and invasion.

Oncogene·2011
Same author

Astrogliosis and behavioral changes in mice lacking the neutral cysteine protease bleomycin hydrolase.

Neuroscience·2007
Same author

Discovery and biological evaluation of a new family of potent inhibitors of the dual specificity protein phosphatase Cdc25.

Journal of medicinal chemistry·2001
Same author

Involvement of Cdc25A phosphatase in Hep3B hepatoma cell growth inhibition induced by novel K vitamin analogs.

Cancer research·2001
Same author

New inhibitors of the thioredoxin-thioredoxin reductase system based on a naphthoquinone spiroketal natural product lead.

Bioorganic & medicinal chemistry letters·2001
Same author

Small molecule inhibitors of dual specificity protein phosphatases.

Oncogene·2001

Cdc25B protein subtypes exhibit distinct subcellular localizations, influencing cell cycle regulation. Cdc25B2 moves to the nucleus during cell division, a process regulated by phosphorylation and DNA damage.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The dual specificity phosphatase and oncogene Cdc25B is crucial for the G2/M cell cycle checkpoint.
  • Mechanisms regulating Cdc25B activity and function, particularly its subcellular localization, are not fully understood.

Purpose of the Study:

  • To investigate the subcellular localization of Cdc25B2 and Cdc25B3 splice variants in live cells.
  • To explore the regulatory mechanisms, including phosphorylation and DNA damage, influencing Cdc25B2 localization and its role in cell cycle control.

Main Methods:

  • Utilized live-cell imaging of green fluorescent protein (GFP)-tagged Cdc25B2 and Cdc25B3.
  • Applied chemical treatments (vanadate, okadaic acid, etoposide) to assess regulatory pathway involvement.
  • Observed protein redistribution in response to cell cycle progression and DNA damage.

Related Experiment Videos

Main Results:

  • Cdc25B2 exhibited cell cycle-dependent nuclear translocation from G1 to G2/M phase, while Cdc25B3 remained diffusely localized.
  • Vanadate treatment disrupted the cell cycle-dependent localization of Cdc25B2, suggesting phosphorylation-dependent regulation.
  • Etoposide inhibited Cdc25B2 nuclear import during S phase, indicating a DNA damage-induced sequestration mechanism.
  • A 41-amino acid N-terminal insert in Cdc25B3 acts as an inhibitory determinant for subcellular redistribution.

Conclusions:

  • Distinct spatial distribution patterns of Cdc25B subtypes exist within cells.
  • Subcellular redistribution of Cdc25B2 is a regulated process potentially critical for G2/M checkpoint function.
  • The N-terminal insert in Cdc25B3 plays a key role in regulating its localization and function.