Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

20.7K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
20.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The scope and impact of original clinical research by Hong Kong public healthcare professionals.

Hong Kong medical journal = Xianggang yi xue za zhi·2025
Same author

Search for a Neutron Dark Decay in ^{6}He.

Physical review letters·2024
Same author

Distinct organization of adaptive immunity in the long-lived rodent Spalax galili.

Nature aging·2023
Same author

Emergency cricothyroidotomy and conversion tracheostomy in a patient with COVID-19: a case report.

Hong Kong medical journal = Xianggang yi xue za zhi·2021
Same author

GWAS Identifies New Loci for Painful Temporomandibular Disorder: Hispanic Community Health Study/Study of Latinos.

Journal of dental research·2017
Same author

Painful Temporomandibular Disorder: Decade of Discovery from OPPERA Studies.

Journal of dental research·2016
Same journal

Clinical Europium fluorescent based lectin assays for mucin O-glycomics.

Methods in enzymology·2026
Same journal

A dual-color FRET assay for detection and quantitative analysis of O-glycopeptidases.

Methods in enzymology·2026
Same journal

Evolutionary genetic approaches to analyze mucins.

Methods in enzymology·2026
Same journal

Ex vivo imaging and enzymatic analysis of intestinal mucus.

Methods in enzymology·2026
Same journal

Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues.

Methods in enzymology·2026
Same journal

Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ.

Methods in enzymology·2026
See all related articles

Related Experiment Video

Updated: Jan 19, 2026

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.9K

Suppression subtractive hybridization: a versatile method for identifying differentially expressed genes.

L Diatchenko1, S Lukyanov, Y F Lau

  • 1CLONTECH Laboratories, Inc., Palo Alto, California 94303-4230, USA.

Methods in Enzymology
|June 1, 1999
PubMed
Summary
This summary is machine-generated.

Suppression subtractive hybridization (SSH) is a novel method that efficiently generates subtracted cDNA libraries. This technique normalizes and subtracts sequences in one step, improving the identification of low-abundance, differentially expressed genes for various genetic studies.

More Related Videos

Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

9.1K
Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
11:06

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

6.5K

Related Experiment Videos

Last Updated: Jan 19, 2026

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.9K
Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

9.1K
Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
11:06

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

6.5K

Area of Science:

  • Molecular Biology
  • Genomics
  • Gene Expression Analysis

Background:

  • Differential gene expression analysis is crucial for understanding biological processes and disease.
  • Traditional methods for generating subtracted cDNA libraries can be time-consuming and inefficient.
  • There is a need for streamlined techniques to identify low-abundance, differentially expressed genes.

Purpose of the Study:

  • To introduce and detail a new method, suppression subtractive hybridization (SSH), for generating normalized and subtracted cDNA libraries.
  • To demonstrate the efficiency and applicability of SSH in identifying differentially expressed genes.
  • To provide protocols for SSH library construction and differential screening.

Main Methods:

  • SSH combines suppression PCR, normalization, and subtraction into a single procedure.
  • Normalization equalizes cDNA abundance within the target population.
  • Subtraction removes common sequences between target and driver populations.

Main Results:

  • SSH generates normalized subtracted cDNA libraries in a single round of hybridization.
  • The method significantly increases the probability of isolating low-abundance differentially expressed cDNAs.
  • SSH simplifies the analysis of subtracted libraries and is applicable to various genetic studies.

Conclusions:

  • SSH is a highly effective and efficient method for constructing subtracted cDNA libraries.
  • The technique facilitates the identification of disease, developmental, and tissue-specific genes.
  • SSH offers a powerful tool for molecular genetic and positional cloning studies.