Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene repair using chimeric RNA/DNA oligonucleotides.

B T Kren1, R Metz, R Kumar

  • 1Department of Medicine, University of Minnesota Medical School, Minneapolis, USA.

Seminars in Liver Disease
|June 1, 1999
PubMed
Summary

Researchers developed a novel DNA editing strategy using chimeric oligonucleotides to precisely correct genetic mutations. This method harnesses the cell's natural repair systems for potential gene therapy applications in genetic diseases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Liver development is restored by blastocyst complementation of HHEX knockout in mice and pigs.

Stem cell research & therapy·2021
Same author

Aberrant MEK5/ERK5 signalling contributes to human colon cancer progression via NF-κB activation.

Cell death & disease·2015
Same author

Use of vibrational spectroscopy in defining the role of clathrin in coated vesicle formation.

Biophysical journal·2009
Same author

Modulation of hepatocyte apoptosis: cross-talk between bile acids and nuclear steroid receptors.

Current medicinal chemistry·2006
Same author

p53 dephosphorylation and p21(Cip1/Waf1) translocation correlate with caspase-3 activation in TGF-beta1-induced apoptosis of HuH-7 cells.

Apoptosis : an international journal on programmed cell death·2004
Same author

The role of retinoblastoma protein in apoptosis.

Apoptosis : an international journal on programmed cell death·2003

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Site-specific genomic DNA alteration is crucial for gene repair.
  • Oligonucleotides with RNA/DNA hybrids show enhanced activity in homologous pairing compared to DNA duplexes.

Purpose of the Study:

  • To develop an experimental strategy for site-specific genomic DNA alteration.
  • To investigate the DNA repair mechanisms involved in this process.

Main Methods:

  • Design of chimeric oligonucleotides with RNA/DNA hybrid regions, thymidine hairpin caps, and a single-strand break.
  • Utilizing the cell's endogenous DNA repair system to correct a designed single base pair mismatch.
  • Studying repair mechanisms in mammalian cell-free extracts and bacterial systems.

Related Experiment Videos

Main Results:

  • The chimeric molecules effectively altered single nucleotides in episomal and genomic DNA in cell culture and in situ.
  • A mismatch correction pathway distinct from homologous recombination was identified.
  • Demonstrated the potential for precise gene editing.

Conclusions:

  • The developed strategy offers a powerful approach for gene repair.
  • This method holds promise for treating genetic diseases caused by point mutations, particularly hepatic genetic diseases.