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[Inhibition of peritoneal bacterial adhesion using oligosaccharides. An experimental model of peritonitis in rats]
I Sielezneff1, M N Mallet, P Berthezene
1Service de chirurgie digestive et générale, hôpital Sainte-Marguerite, Marseille, France.
Background:
Peritoneal colonization is a crucial event in the pathogenesis of peritonitis and its local complications. Adherence to the serosal mesothelium is mediated in a number of microorganisms derived from the digestive tract (especially E. coli) by type-1 fimbriae which have an oligosaccharide specificity.
Purpose:
To evaluate the effect of repeated peritoneal washes with saline solution and oligosaccharides on E. coli peritoneal adherence in a rat peritonitis model.
Methods:
Sixty rats were randomized in 3 groups of 20. E. coli was inoculated at a constant concentration of 10(8)/mL per 100 g of weight. Then, peritoneal washes were achieved daily during three consecutive days (D1, D2, D3), with saline solution in Group I (control group), Methyl alpha-D-Mannoside (MADM) in Group II, and p-Nitro-phenyl alpha-D-Mannoside (pNADM) in Group III. Peritoneal samples were obtained before and after lavage at D1, D2, and D3. Microbial recovery was expressed as cfu/mg of tissue, and converted into a percentage of the initial value. A 10% threshold defined efficiency of the wash (inhibition of adherence for 90% of bacteries).
Results:
Compared with data from Group I, E. coli peritoneal adherence was significantly lower after washes in Group III (D1: p = 0.03; D2: p = 0.009; D3: p = 0.003). Repeated washes were more efficient in Group III than in Group II (D1: p = 0.1; D2: p = 0.5; D3: p = 0.001).
Conclusion:
These results suggest that the addition of oligosaccharides, especially of pNADM, reduces the peritoneal adherence of E. coli when a peritoneal wash is performed for peritonitis.
Insights
Repeated peritoneal washes with p-Nitro-phenyl alpha-D-Mannoside (pNADM) significantly reduced E. coli adherence in a rat peritonitis model. This suggests pNADM is effective in preventing bacterial colonization during peritonitis treatment.
Area of Science:
- Microbiology
- Pathogenesis
- Surgical Infection
Background:
- Peritoneal colonization by microorganisms, particularly E. coli, is key in peritonitis development.
- Type-1 fimbriae mediate bacterial adherence to the serosal mesothelium via oligosaccharide specificity.
Purpose of the Study:
- To assess the impact of repeated peritoneal washes with saline and specific oligosaccharides on E. coli peritoneal adherence.
- To evaluate the efficacy of Methyl alpha-D-Mannoside (MADM) and p-Nitro-phenyl alpha-D-Mannoside (pNADM) in reducing bacterial adherence in a rat peritonitis model.
Main Methods:
- Sixty rats were divided into three groups receiving daily peritoneal washes over three days.
- Groups were treated with saline (control), MADM, or pNADM.
- Bacterial adherence was quantified by microbial recovery (cfu/mg tissue) before and after washes, with 90% inhibition defining wash efficiency.
Main Results:
- Peritoneal washes with pNADM (Group III) significantly reduced E. coli adherence compared to saline (Group I) on all study days (p < 0.05).
- pNADM washes were more effective than MADM washes (Group II) by day 3 (p = 0.001).
Conclusions:
- The addition of oligosaccharides, particularly pNADM, effectively reduces E. coli peritoneal adherence.
- These findings support the use of pNADM in peritoneal washes as an adjunctive therapy for peritonitis to minimize bacterial colonization.
