[Effects of amniotic fluid on the development of neoplastic tissue in organotypic culture]

In previous researches, it has been observed that the human amniotic fluid presents in vitro an antineoplasic action. 75% of the examined amniotic fluids contain an anti HCG principle. In order to evaluate if the effect of amniotic fluid is due to this principle, cultures of tumoral tissue have been performed, with the Wolff and Wolff's method, using media containing either positive amniotic fluid or negative amniotic fluid. Results obtained with positive amniotic fluid have confirmed the previous observations. A complete degeneration of both tumoral tissue and mesonephros was observed when the culture medium was enriched with negative amniotic fluid.