Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Improved immunoradiometric assay for plasma renin.

J Deinum1, F H Derkx, M A Schalekamp

  • 1Department of Internal Medicine I, University Hospital Dijkzigt, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands. deinum@inw1.azr.nl

Clinical Chemistry
|June 3, 1999
PubMed
Summary

This study modified an immunoassay (IRMA) for measuring renin by increasing the temperature and decreasing incubation time. The new method accurately measures renin without overestimating it due to prorenin.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

On the origin of cerebral small vessel disease: MRI markers of cSVD in young adults with hypertension.

Cerebral circulation - cognition and behavior·2025
Same author

The position of renal denervation in treatment of hypertension: an expert consensus statement.

Netherlands heart journal : monthly journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation·2022
Same author

Volumetric evaluation of CT images of adrenal glands in primary aldosteronism.

Journal of endocrinological investigation·2021
Same author

Pregnancy and phaeochromocytoma/paraganglioma: clinical clues affecting diagnosis and outcome - a systematic review.

BJOG : an international journal of obstetrics and gynaecology·2020
Same author

Fibrinolysis inhibitors in plaque stability: a morphological association of PAI-1 and TAFI in advanced carotid plaque.

Journal of thrombosis and haemostasis : JTH·2017
Same author

Evaluating the learning curve for retroperitoneoscopic adrenalectomy in a high-volume center for laparoscopic adrenal surgery.

Surgical endoscopy·2016

Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Assay Development

Background:

  • Immunoradiometric assays (IRMA) for renin can overestimate results in plasmas with high prorenin levels.
  • This overestimation may stem from prorenin converting to an active form during the assay, rather than antibody cross-reactivity.

Purpose of the Study:

  • To modify an existing renin IRMA to eliminate interference from prorenin.
  • To improve the accuracy and efficiency of plasma renin activity measurement.

Main Methods:

  • The assay temperature was increased from 22°C to 37°C.
  • Incubation time was reduced from 24 hours to 6 hours.
  • The modified IRMA was validated against an enzyme-kinetic assay.

Main Results:

Related Experiment Videos

  • The modified IRMA eliminated the co-measurement of prorenin as renin.
  • Assay precision and accuracy were comparable to the original IRMA.
  • The modified assay showed close agreement with enzyme-kinetic measurements and was not affected by angiotensinogen levels.

Conclusions:

  • The modified IRMA, conducted at 37°C, effectively prevents prorenin interference.
  • The new method retains the analytical strengths of the previous IRMA.
  • The modified assay offers a faster and more accurate measurement of plasma renin activity.