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Related Experiment Videos

EGFr and c-erB2mRNA expression in urinary bladder superficial carcinomas.

Bonin1, Lissiani, Maffezzini

  • 1Department of Pathology, University of Trieste, Trieste, 34100,Italy.

Advances in Clinical Pathology : the Official Journal of Adriatic Society of Pathology
|April 1, 1997
PubMed
Summary

Predicting superficial bladder cancer behavior is challenging. While k-ras oncogene expression may indicate aggressiveness, epidermal growth factor receptor (EGFr) shows higher expression in less aggressive superficial tumors.

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Area of Science:

  • Uro-oncology
  • Molecular diagnostics
  • Cancer biomarkers

Background:

  • Superficial transitional carcinomas of the urinary bladder exhibit unpredictable biological behavior.
  • Differentiating aggressive, infiltrative tumors from non-aggressive ones is crucial for patient management.

Purpose of the Study:

  • To investigate the mRNA expression of epidermal growth factor receptor (EGFr), c-erbB2, and k-ras oncogene.
  • To correlate gene expression with the clinical outcomes of superficial urinary bladder transitional carcinomas.

Main Methods:

  • Relative mRNA quantitation using reverse transcription polymerase chain reaction (RT-PCR).
  • Analysis of formalin-fixed, paraffin-embedded tumor tissues from 23 patients.
  • Correlation of gene expression with follow-up data on tumor recurrence patterns (superficial vs. infiltrative).

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Main Results:

  • Epidermal growth factor receptor (EGFr) mRNA was generally higher in tumors with superficial recurrences.
  • c-erbB2 mRNA levels showed no significant difference between aggressive and less aggressive tumor groups.
  • k-ras oncogene mRNA expression was elevated in cases with more infiltrative tumor relapses.

Conclusions:

  • No single marker definitively predicts prognosis for superficial bladder transitional carcinoma in this preliminary study.
  • k-ras oncogene expression shows some association with tumor aggressiveness.
  • Elevated EGFr expression in superficial carcinomas may indicate a less infiltrative behavior.