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Viability studies on actinomycetes.

A Taddei1, M M Tremarias, C Hartung de Capriles

  • 1Departamento de Química, Universidad Simón Bolívar, Caracas, Venezuela. ataddei@usb.ve

Mycopathologia
|June 3, 1999
PubMed
Summary
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Long-term storage of Actinomycetes under paraffin oil maintained viability for decades. Some strains retained characteristics and biological activity, demonstrating effective preservation methods for microbial collections.

Area of Science:

  • Microbiology
  • Actinobacteria Research

Background:

  • Long-term preservation of microbial cultures is crucial for maintaining biodiversity and accessibility.
  • Actinomycetes, particularly Streptomyces species, are vital sources of antibiotics and other bioactive compounds.
  • Traditional preservation methods like paraffin oil overlay have been used for decades, but their long-term efficacy requires validation.

Purpose of the Study:

  • To assess the viability and characteristics of Actinomycetes strains preserved for 10-30 years under paraffin oil.
  • To evaluate the morphological and physiological stability of these long-term stored strains.
  • To determine the retained biological activity of recovered Actinomycetes strains.

Main Methods:

  • Eighty-nine Actinomycetes strains from the Lorenzo De Montemayor collection were revived after 10-30 years of storage.

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  • Storage methods included paraffin oil overlay and distilled water.
  • Viability, morphology, physiology, and biological activity (antimicrobial assays) were assessed post-revival.

    • Main Results:

    • A high percentage of strains remained viable after 28-30 years without subculturing.
    • Streptomyces violaceoruber maintained pigment production after 28 years.
    • Streptomyces lavendulae exhibited significant growth inhibition against Staphylococcus aureus and Bacillus subtilis.

    Conclusions:

    • Paraffin oil overlay is a highly effective method for long-term preservation of Actinomycetes viability and key characteristics.
    • Microbial collections stored for extended periods can yield strains with retained biological activities.
    • This study validates the utility of long-term cryopreservation techniques for Actinomycetes research and bioprospecting.