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Involvement of diazepam binding inhibitor and its fragment octadecaneuropeptide in social isolation stress-induced
1Department of Pharmacology, Research Institute for Wakan-Yaku (Oriental Medicines), Toyama Medical and Pharmaceutical University, Japan.
Life Sciences
|June 3, 1999
Summary
Diazepam binding inhibitor (DBI) and octadecaneuropeptide (ODN) may influence sleep duration. Social isolation in mice reduced pentobarbital sleep, an effect modulated by DBI/ODN and flumazenil.
Area of Science:
- Neuroscience
- Pharmacology
- Behavioral Science
Background:
- Diazepam binding inhibitor (DBI) and its fragment, octadecaneuropeptide (ODN), are endogenous ligands for benzodiazepine (BZD) receptors.
- DBI and ODN act as inverse BZD receptor agonists, potentially influencing stress responses and sleep.
- Previous research linked social isolation stress to reduced pentobarbital sleep, possibly via inverse BZD receptor agonists.
Purpose of the Study:
- To investigate the role of DBI and ODN in the social isolation-induced decrease in pentobarbital sleep in mice.
- To determine if DBI and ODN affect pentobarbital-induced sleep duration in group-housed versus socially isolated mice.
- To explore the interaction between DBI, ODN, and flumazenil (a BZD receptor antagonist) in modulating pentobarbital sleep.
Main Methods:
- Administered diazepam binding inhibitor (DBI) and octadecaneuropeptide (ODN) intracerebroventricularly (i.c.v.) to group-housed and socially isolated mice.
- Assessed the effects of DBI and ODN on pentobarbital-induced sleeping time (50 mg/kg, i.p.).
- Investigated the modulatory effects of flumazenil (i.c.v.) on pentobarbital sleep and the actions of DBI and ODN.
Main Results:
- Socially isolated mice exhibited significantly shorter pentobarbital sleep durations compared to group-housed mice.
- Intracerebroventricular administration of DBI and ODN dose-dependently reduced pentobarbital sleep in group-housed mice but had no effect in socially isolated mice.
- Flumazenil reversed the reduced sleep time in socially isolated mice to levels seen in group-housed mice, and its effects were modulated by DBI and ODN.
Conclusions:
- Changes in the activity of Diazepam binding inhibitor (DBI) and/or octadecaneuropeptide (ODN) contribute to the social isolation-induced reduction in pentobarbital's hypnotic effect.
- DBI and ODN appear to be involved in the altered sleep regulation observed following social isolation stress.
- The interplay between DBI/ODN and benzodiazepine receptors is crucial in mediating the effects of social isolation on sleep.