Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Prognostic impact of lymphovascular invasion and efficacy of adjuvant therapy in patients with non-metastatic clear cell renal cell carcinoma: a single-center retrospective cohort study].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2026
Same author

Competitive enzyme-linked immunosorbent assay to quantify hepcidin in horse serum.

Journal of equine veterinary science·2026
Same author

[Prognostic value of lymph node dissection in radical nephrectomy for cN0M0 renal cell carcinoma].

Zhonghua zhong liu za zhi [Chinese journal of oncology]·2026
Same author

Early detection of renal cell carcinoma: a novel cell-free DNA fragmentomics-based liquid biopsy assay.

ESMO open·2025
Same author

[Dynamic distribution and clearance of <sup>99m</sup>Tc-DTPA in brain extracellular space].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2025
Same author

The cardio-renal-metabolic role of the nod-like receptor protein-3 and senescence-associated secretory phenotype in early sodium/glucose cotransporter-2 inhibitor therapy in people with diabetes who have had a myocardial infarction.

Diabetic medicine : a journal of the British Diabetic Association·2025

Related Experiment Video

Updated: Jul 29, 2026

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Accelerated assembly of G-quadruplex structures by a small molecule.

H Han1, C L Cliff, L H Hurley

  • 1Institute for Cellular and Molecular Biology, Division of Medicinal Chemistry, Drug Dynamics Institute, The University of Texas at Austin 78712, USA.

Biochemistry
|June 3, 1999
PubMed
Summary

A small molecule, PIPER, acts as a novel driver for G-quadruplex DNA assembly, significantly accelerating the formation of these crucial secondary structures. This discovery offers new insights into G-quadruplex formation and potential therapeutic strategies.

More Related Videos

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Related Experiment Videos

Last Updated: Jul 29, 2026

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • DNA oligomers with G-rich repeats form G-quadruplex structures in the presence of alkali cations.
  • G-quadruplex formation is typically a slow process, though some proteins can act as chaperones.
  • Small molecules are increasingly being investigated for their roles in modulating nucleic acid structures.

Purpose of the Study:

  • To investigate the role of a perylene derivative, PIPER, in the assembly of G-quadruplex structures.
  • To determine if small ligands can act as drivers for polynucleotide secondary structure assembly.
  • To elucidate the kinetics and mechanism by which PIPER influences G-quadruplex formation.

Main Methods:

  • Gel-shift experiments were used to monitor the association of DNA oligomers.
  • Kinetic analysis was performed to determine the order and rate of oligomer dimerization.
  • The effect of varying PIPER concentrations on G-quadruplex assembly was quantified.

Main Results:

  • PIPER dramatically accelerates the association of DNA oligomers into di- and tetrameric G-quadruplexes.
  • PIPER shifts the oligomer dimerization kinetics from second order to first order.
  • 10 microM PIPER accelerates the assembly of dimeric G-quadruplexes approximately 100-fold.

Conclusions:

  • PIPER is the first small ligand identified as a driver for G-quadruplex DNA assembly.
  • The acceleration of G-quadruplex formation by PIPER has implications for understanding biological effects of G-quadruplex-interactive agents.
  • This finding opens new avenues for designing small molecules to control nucleic acid secondary structures.