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Related Experiment Videos

Genetic diversity among Trichophyton mentagrophytes isolates using random amplified polymorphic DNA method.

G Kac1, M E Bougnoux, M Feuilhade De Chauvin

  • 1Laboratoire de Parasitologie-Mycologie, Hôpital Saint-Louis, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France.

The British Journal of Dermatology
|June 3, 1999
PubMed
Summary

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Genetic diversity in Trichophyton mentagrophytes was analyzed using random amplified polymorphic DNA (RAPD). This dermatophyte species shows limited genetic variation, with some strains appearing homogeneous across multiple lesions.

Area of Science:

  • Medical Mycology
  • Molecular Biology
  • Dermatology

Background:

  • Trichophyton mentagrophytes is a widespread dermatophyte species with anthropophilic (var. interdigitale) and zoophilic (var. mentagrophytes) varieties.
  • Understanding the genetic diversity of T. mentagrophytes is crucial for epidemiological studies and treatment strategies.

Purpose of the Study:

  • To investigate the genetic diversity within the Trichophyton mentagrophytes complex using clinical isolates.
  • To assess the utility of the random amplified polymorphic DNA (RAPD) method for differentiating T. mentagrophytes strains.

Main Methods:

  • Analysis of 46 clinical isolates of T. mentagrophytes from diverse geographical origins (Europe, Africa, South America).
  • Application of the random amplified polymorphic DNA (RAPD) technique with 15 different primers.

Related Experiment Videos

  • Clustering of isolates based on DNA polymorphism patterns.

    • Main Results:

    • Only one out of 15 primers revealed DNA polymorphism, yielding 23 distinct patterns grouped into three clusters.
    • No geographical clustering was observed; however, all T. mentagrophytes var. mentagrophytes isolates shared the same pattern.
    • Identical strains from multiple lesions in seven patients suggested population homogeneity, while dissimilar strains from cohabiting patients argued against direct transmission.

    Conclusions:

    • The Trichophyton mentagrophytes complex exhibits limited genetic diversity among the studied clinical isolates.
    • Random amplified polymorphic DNA (RAPD) is a viable method for demonstrating genetic diversity within T. mentagrophytes.
    • Findings suggest potential for strain homogeneity in certain clinical presentations and challenge direct person-to-person transmission in specific cohabiting cases.