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An improved micro-method for obtaining chromosome preparations from individual haemopoietic colonies
M A Kadhim1, D A Pocock, S A Lorimore
1Radiation and Genome Stability Unit, Medical Research Council, Harwell, Oxfordshire.
British Journal of Haematology
|June 3, 1999
Abstract:
A robust method for obtaining chromosome preparations from individual haemopoietic colonies in semi-solid media is described. The accumulation of metaphases and the hypotonic treatment of cells were carried out in the culture dish and individual colonies were transferred onto poly-L-lysine-treated slides and fixed stepwise prior to staining. The technique produced high yields of well-spread metaphases facilitating clonal cytogenetic analysis.