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Updated: Oct 20, 2026

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria
Published on: November 10, 2021
Crystal structure of C-phycocyanin from Cyanidium caldarium provides a new perspective on phycobilisome assembly
B Stec1, R F Troxler, M M Teeter
1Department of Chemistry, Merkert Chemistry Building, Boston College, Chestnut Hill, Massachusetts 02167, USA. stec@bioc.rice.edu
Abstract:
The crystal structure of the light-harvesting protein phycocyanin from the cyanobacterium Cyanidium caldarium with novel crystal packing has been solved at 1.65-A resolution. The structure has been refined to an R value of 18.3% with excellent backbone and side-chain stereochemical parameters. In crystals of phycocyanin used in this study, the hexamers are offset rather than aligned as in other phycocyanins that have been crystallized to date. Analysis of this crystal's unique packing leads to a proposal for phycobilisome assembly in vivo and for a more prominent role for chromophore beta-155. This new role assigned to chromophore beta-155 in phycocyanin sheds light on the numerical relationships among and function of external chromophores found in phycoerythrins and phycoerythrocyanins.
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