Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Creation and evaluation of a gender diversity focused cultural competency training for phlebotomists: Study protocol for a randomized controlled trial.

Heliyon·2025
Same author

An in vitro study of the effect of the optimal irrigation solution conditions during canine articular surgery.

Veterinary research communications·2019
Same author

Pilot feasibility randomized clinical trial of negative-pressure wound therapy versus usual care in patients with surgical wounds healing by secondary intention.

BJS open·2018
Same author

Homeless.

The Hospital·2018
Same author

Metropolitan Association for Befriending Young Servants.

The Hospital·2018
Same author

A multicentre, clinical evaluation of a hydro-responsive wound dressing: the Glasgow experience.

Journal of wound care·2017

Related Experiment Video

Updated: Jul 12, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Discrimination of DNA hybridization using chemical force microscopy.

L T Mazzola1, C W Frank, S P Fodor

  • 1Department of Chemistry, Stanford University, Stanford, California 94305, USA.

Biophysical Journal
|June 4, 1999
PubMed
Summary

Chemical force microscopy (CFM) measured DNA hybridization forces. Complementary DNA sequences showed stronger friction and adhesion, indicating sequence-specific interactions on surfaces.

More Related Videos

Ensemble Force Spectroscopy by Shear Forces
07:30

Ensemble Force Spectroscopy by Shear Forces

Published on: July 26, 2022

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
10:11

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

Published on: July 26, 2024

Related Experiment Videos

Last Updated: Jul 12, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Ensemble Force Spectroscopy by Shear Forces
07:30

Ensemble Force Spectroscopy by Shear Forces

Published on: July 26, 2022

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
10:11

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

Published on: July 26, 2024

Area of Science:

  • Biophysics
  • Surface Science
  • Nanotechnology

Background:

  • Atomic force microscopy (AFM) enables probing molecular interactions.
  • Chemical force microscopy (CFM) uses chemically modified AFM tips to detect molecular recognition.
  • AFM tips can be functionalized with ligands to identify specific binding partners on surfaces.

Purpose of the Study:

  • To investigate the use of CFM for probing sequence-specific interactions between DNA oligonucleotides.
  • To measure the mechanical forces, specifically friction and adhesion, during DNA hybridization.
  • To correlate measured forces with the molecular affinity and hydrogen bonding between complementary DNA strands.

Main Methods:

  • Functionalizing an AFM cantilever with a DNA oligonucleotide (3 omino-CAGTTCTACGATGGCAAGTC).
  • Scanning patterned substrates containing complementary and noncomplementary DNA sequences with the functionalized AFM tip.
  • Measuring friction and adhesion forces between the DNA-modified tip and the surface-bound DNA.

Main Results:

  • A strong friction interaction was observed between the DNA-modified tip and surface-bound DNA.
  • Complementary DNA sequences exhibited significantly stronger friction and adhesion (rupture force) compared to noncomplementary sequences.
  • Friction forces correlated directly with adhesion forces, suggesting a common origin in molecular interactions.

Conclusions:

  • CFM can effectively detect sequence-specific hybridization between surface-bound DNA and a DNA-modified AFM tip.
  • The observed friction and adhesion forces are indicative of DNA hybridization, driven by hydrogen bond formation.
  • This study demonstrates CFM as a powerful tool for analyzing molecular recognition and mechanical properties of DNA at the nanoscale.