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Related Experiment Video

Updated: Jul 18, 2026

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes

Published on: February 8, 2016

Inhibition by cAMP of calcium-activated chloride currents in cultured Sertoli cells from immature testis.

N Lalevée¹, M Joffre

¹Laboratoire de Physiologie des Régulations Cellulaires, UMR 6558, UFR Sciences, 40 Avenue du Recteur Pineau 86022-Poitiers-Cedex, France.

The Journal of Membrane Biology

|June 4, 1999

Summary

This study identified a calcium-dependent chloride current (ClCa) in rat Sertoli cells. Cyclic AMP (cAMP) inhibits this current, suggesting a role in regulating Sertoli cell function and fluid secretion.

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Area of Science:

Reproductive Biology
Cell Physiology
Ion Transport

Background:

Sertoli cells in immature rat testis play crucial roles in spermatogenesis.
Understanding ion channel activity in Sertoli cells is key to elucidating testicular fluid regulation.

Purpose of the Study:

To characterize the calcium-dependent chloride current (ClCa) in cultured rat Sertoli cells.
To investigate the regulation of ClCa by intracellular calcium and cyclic AMP (cAMP).

Main Methods:

Whole-cell patch-clamp technique was employed on cultured immature rat Sertoli cells.
Ionic currents were measured under varying intracellular calcium concentrations and in the presence of specific inhibitors (DIDS, 9-AC).
The effects of ATP, ionomycin, and cAMP on ClCa were assessed.

Main Results:

A Ca2+-dependent Cl- current (ClCa) was identified, sensitive to DIDS and 9-AC but not TEA.
ClCa magnitude was directly dependent on intracellular Ca2+ levels and not directly modulated by ATP.
cAMP and its analogues significantly inhibited ClCa, suggesting inhibition by cAMP-dependent protein kinase.

Conclusions:

ClCa in Sertoli cells is regulated by intracellular calcium and inhibited by cAMP.
These findings challenge exocrine secretion models and support a hyperpolarizing role for cAMP in Sertoli cells.
This suggests Sertoli cells release a primary fluid low in chloride but rich in bicarbonate.