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[Vaccine development for P. aeruginosa: based on antigen cloning of clinical vaccines][
1Abteilung Allgemeine Chirurgie mit Poliklinik, Chirurgische Universitätsklinik Freiburg.
Zentralblatt Fur Chirurgie
|June 4, 1999
Abstract:
For the development of a clinical vaccine against P. aeruginosa infections we cloned the genes for the main outer membrane proteins F and I of P. aeruginosa and characterized protective epitopes by monoclonal antibodies. A recombinant hybrid protein (OprFaa190-342-OprIaa21-83) was expressed in E. coli which represents the protective epitopes. The vaccine showed to be highly protective in mice against experimental P. aeruginosa infections. A phase I trial in human volunteers showed that the vaccine is well tolerated and that high antibody titers against P. aeruginosa were induced.