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Release of outer membrane vesicles from Bordetella pertussis
D Hozbor1, M E Rodriguez, J Fernández
1Centro de Investigación y Desarrollo en Fermentaciones Industriales, CONICET Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina.
Current Microbiology
|June 4, 1999
Summary
Researchers investigated Bordetella pertussis outer membrane vesicles (OMVs). These OMVs contain adenylate cyclase-hemolysin (AC-Hly) and protective immunogens, suggesting their potential for acellular pertussis vaccines.
Area of Science:
- Microbiology
- Immunology
- Vaccine Development
Background:
- Bordetella pertussis is the causative agent of whooping cough.
- Outer membrane vesicles (OMVs) are potential vaccine candidates.
- Understanding OMV production is crucial for vaccine development.
Purpose of the Study:
- To investigate the production of Bordetella pertussis outer membrane vesicles (OMVs).
- To characterize the protein and lipopolysaccharide (LPS) content of these OMVs.
- To assess the potential of OMVs as a basis for acellular pertussis vaccines.
Main Methods:
- Culturing B. pertussis in Stainer-Scholte liquid medium.
- Inducing vesicle formation via sonication of bacterial suspensions.
- Immunoblot analysis to identify OMV components, including adenylate cyclase-hemolysin (AC-Hly) and LPS.
Main Results:
- Numerous OMVs were observed during bacterial growth and induced by sonication.
- OMVs were confirmed to contain AC-Hly and LPS.
- AC-Hly was identified as an integral component of the OMVs.
Conclusions:
- B. pertussis produces OMVs naturally and via artificial induction.
- OMVs contain key components like AC-Hly and LPS.
- These OMVs possess protective immunogens, indicating their promise for acellular pertussis vaccine development.