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Imaging epicardial oxygen.

C H Barlow¹, D A Rorvik, J J Kelly

¹Barlow Scientific, Inc., Olympia, WA, USA. barlowc@evergreen.edu

Annals of Biomedical Engineering

|June 4, 1999

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This study developed a novel imaging system to visualize oxygen and metabolic changes in rat hearts. The system reveals how local ischemia and perfusion impact cardiac respiration and energy status.

Area of Science:

Physiology
Biophysics
Biochemistry

Background:

Understanding cardiac respiration is crucial for analyzing heart function under varying workloads and substrate availability.
Regional oxygen and metabolic status are key indicators of cardiac health and response to physiological challenges.

Purpose of the Study:

To develop and utilize a novel fluorescence/phosphorescence imaging system for real-time measurement of epicardial oxygen and mitochondrial NADH concentrations in perfused rat hearts.
To investigate regional changes in cardiac oxygen and metabolic status in response to induced local ischemia, heterogeneous perfusion, and barbiturate inhibition.

Main Methods:

Development of a fluorescence/phosphorescence imaging system capable of acquiring digital images of oxygen and NADH.
Oxygen imaging based on the phosphorescence quenching of Pd(II) meso-tetra(p-sulfonatophenyl)porphin by dissolved oxygen.

Related Experiment Videos

Simultaneous imaging of epicardial oxygen and mitochondrial NADH fluorescence in isolated perfused rat hearts.

Main Results:

The imaging system successfully captured regional variations in epicardial oxygen and mitochondrial NADH levels.
Images demonstrated distinct gradients of oxygen and NADH across the boundary of locally ischemic and normoxic epicardial tissue.
The study illustrated the complementary information provided by oxygen and NADH images regarding cardiac oxygen supply and demand.

Conclusions:

The developed two-dimensional imaging technique provides a powerful tool for studying regional oxygen supply/demand dynamics in cardiac muscle.
This method allows for detailed analysis of mitochondrial bioenergetic status and its response to altered physiological conditions.
The findings highlight the utility of simultaneous oxygen and NADH imaging for understanding cardiac respiration mechanisms.