Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Optical methods for probing mitochondrial function in brain slices.

T J Sick1, M A Perez-Pinzon

  • 1Department of Neurology, University of Miami School of Medicine, South Campus, Building B, 12500 Southwest 152 Street, Miami, Florida 33177, USA. tsick@neuron.med.miami.edu

Methods (San Diego, Calif.)
|June 5, 1999
PubMed
Summary

Optical methods can probe brain slice mitochondrial function, aiding research into neurological disorders and brain pathophysiology. These techniques measure redox activity and membrane potential, proving useful in studying ischemia-induced mitochondrial dysfunction.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Forebrain ischemia triggers GABAergic system degeneration in substantia nigra at chronic stages in rats.

Cardiovascular psychiatry and neurology·2010
Same author

Post-ischemic activation of protein kinase C ε protects the hippocampus from cerebral ischemic injury via alterations in cerebral blood flow.

Neuroscience letters·2010
Same author

Derangements of post-ischemic cerebral blood flow by protein kinase C delta.

Neuroscience·2010
Same author

Oxygen-conserving reflexes of the brain: the current molecular knowledge.

Journal of cellular and molecular medicine·2009
Same author

Resveratrol pretreatment protects rat brain from cerebral ischemic damage via a sirtuin 1-uncoupling protein 2 pathway.

Neuroscience·2009
Same author

Pretreatment with a single estradiol-17beta bolus activates cyclic-AMP response element binding protein and protects CA1 neurons against global cerebral ischemia.

Neuroscience·2009

Area of Science:

  • Neuroscience
  • Biochemistry
  • Cell Biology

Background:

  • Brain slice preparations are vital for studying brain function and disease.
  • Mitochondrial dysfunction is increasingly implicated in various neurological disorders.
  • Understanding mitochondrial roles in brain health and disease is crucial.

Purpose of the Study:

  • To discuss optical methods for assessing mitochondrial function in brain slices.
  • To highlight techniques for measuring key mitochondrial parameters.
  • To demonstrate the utility of these methods in disease models.

Main Methods:

  • Absorption spectrophotometry to measure mitochondrial redox activity.
  • Spectrofluorometry to assess nicotinamide adenine dinucleotide (NADH) redox state.

Related Experiment Videos

  • Spectrofluorometry with JC-1 dye to evaluate mitochondrial membrane potential.

    • Main Results:

    • The described optical methods effectively probe mitochondrial redox state and membrane potential in brain slices.
    • These techniques were successfully applied to study in vitro ischemia in hippocampal slices.
    • The results demonstrate the feasibility of using optical methods to investigate mitochondrial dysfunction.

    Conclusions:

    • Optical methods offer powerful tools for studying mitochondrial function in brain slices.
    • These techniques are valuable for investigating mitochondrial involvement in neurological disorders, including ischemia.
    • Further application of these methods could advance our understanding of brain pathophysiology.