Related Experiment Videos
Using hippocampal slices to study how aging alters ion regulation in brain tissue
1Geriatric Research, Education, and Clinical Center, Miami VA Medical Center, Miami, Florida 33125, USA. roberts@neuron.med.miami.edu
Methods (San Diego, Calif.)
|June 5, 1999
Abstract:
Aging alters ion regulation in brain tissue. This article describes methods useful for studying such age-related changes in the rat hippocampal slice preparation. Topics considered include (a) selection of appropriate age groups of rats for aging studies, (b) a description of methods for preparing and maintaining hippocampal slices, (c) measurement of intracellular pH with the H+-sensitive dye carboxy-SNARF-1, and (d) measurement of extracellular pH and K+ with cation-selective microelectrodes.