[Platelet adhesion to collagen in whole blood: a simple and rapid quantitative method]

A simple, rapid, and easily reproducible method for quantitative assessment and graphic representation of platelet adhesion of whole blood specimens is proposed. It is based on registration (by means of impedance aggregometer) of changes in the module of complex resistance of an EDTA-stabilized blood specimen by collagen. The amplitude of the process initiated under such conditions directly depends on collagen concentration, weakly decreases if blood is incubated with high concentrations of acetylsalicylic acid (500 micromol/liter), and significantly decreases in the presence of RGD-containing peptides in a concentration of 20-40 micromol/liter). A relatively higher collagen adhesion capacity of blood platelets from coronary patients correlates with their high aggregation activity.