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Detection of bluetongue virus genome segment 6 sequences by RT-PCR
S K Bandyopadhyay1, R S Kataria, A K Tiwari
1National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar, India.
Indian Journal of Experimental Biology
|June 5, 1999
Abstract:
Reverse transcription-PCR (RT-PCR) technique was adopted to amplify a 101 basepair nucleotide sequence of bluetongue virus (BTV) genome segment 6. The specificity of the amplicon was determined by its approximate size in 3% agarose gel electrophoresis, digestion with restriction enzyme MspI, dot-blot hybridization and cycle sequencing. The technique was found to be suitable for detection of bluetongue virus in infected cell culture and clinical samples.