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Isolation and characterization of biologically active lectin from Korean mistletoe, Viscum album var. Coloratum
1College of Pharmacy, Wonkwang University, Iksan, Korea. hslee@wonnms.wonkwang.ac.kr
Cellular and Molecular Life Sciences : CMLS
|June 5, 1999
Abstract:
A mistletoe lectin was isolated from water extracts of Korean mistletoe, a subspecies of Viscum album, grown on Quercus mongolica using CM-Sepharose chromatography followed by an affinity chromatography on a concanavalin A-Sepharose column. The compound proved to be a mistletoe lectin II with D-galactose and N-acetyl-D-galactosamine specificity. Matrix-assisted laser desorption time-of-flight mass spectroscopy showed it to have an average molecular mass of 62.7 kDa and to consist of two subunits of 30.6 kDa and 32.5 kDa. It was a basic protein with isoelectric points of 9.4 and 9.6 by capillary isoelectric focusing and was cytotoxic to Molt4 cell.