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Studies on the characterization of the Rho(D) antigen.

J Litten, R Culpepper, S Bakerman

Biochimica Et Biophysica Acta

|October 3, 1978

Summary

The Rho(D) antigen

Area of Science:

Immunochemistry
Biochemistry
Cell Biology

Background:

The Rho(D) antigen is crucial for red blood cell compatibility.
Understanding its chemical nature aids transfusion medicine and diagnostics.

Purpose of the Study:

To elucidate the chemical characteristics of the Rho(D) antigen.
To identify the molecular components essential for Rho(D) antigen activity.

Main Methods:

Solubilization of Rho(D) antigen from red cell membranes using EDTA and 2-mercaptoethanol.
Partial purification via molecular filtration.
Enzymatic digestion to assess activity changes.

Main Results:

Rho(D) antigen activity, measured by hemagglutination inhibition, was unaffected by phospholipases, phosphatases, neuraminidase, and carboxypeptidases.

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Proteolytic enzymes (pronase, trypsin, chymotrypsin, papain) destroyed Rho(D) activity.

Lipid's role in maintaining activity in intact membranes was confirmed, but not required for solubilized antigen.

Conclusions:

Protein is a critical component of the active determinant of the Rho(D) antigen.
Solubilized Rho(D) antigen's activity is independent of lipids, suggesting a conformational role for lipids in native membranes.