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Examination of Thymic Positive and Negative Selection by Flow Cytometry
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Examination of Thymic Positive and Negative Selection by Flow Cytometry

Published on: October 8, 2012

Functional and phenotypic analysis of thymic B cells: role in the induction of T cell negative selection

I Ferrero1, F Anjuère, P Martín

  • 1Department of Cell Biology, Faculty of Biology, Universidad Complutense, Madrid, Spain.

European Journal of Immunology
|June 8, 1999
PubMed

The phenotype of mouse thymic B cells and their capacity to induce T cell negative selection in vitro were analyzed. Thymic B cells expressed B cell markers such as IgM, Fc gamma receptor, CD44, heat-stable antigen, LFA-1 and CD40. In addition, they were positive for the activation molecule CD69 and displayed high levels of B7-2. Although thymic B cells expressed CD5 on their surface, no CD5-specific mRNA was detected. Moreover, thymic B cells induced a stronger deletion of TCR-transgenic (TG) thymocytes than splenic B cells, which had low CD69 and B7-2 levels. Interestingly, CD40-activated splenic B cells up-regulated CD69 and B7-2 and acquired a capacity to induce T cell deletion comparable to that of thymic B cells. Moreover, thymic B cells from CD40-deficient mice displayed lower CD69 and B7-2 levels than control thymic B cells, and lower capacity to induce the deletion of TCR TG thymocytes. These results support the hypothesis that CD40-mediated activation of thymic B cells determines a high efficiency of antigen presentation, suggesting that within the thymus B cells may play an important role in the elimination of autoreactive thymocytes.

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