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Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching
Published on: June 30, 2010
Vascular development in the mouse embryonic pancreas and lung
K L Colen1, C A Crisera, M I Rose
1Department of Surgery, New York University Medical Center, NY 10016, USA.
Journal of Pediatric Surgery
|June 8, 1999
Summary
This study investigated angiopoietin-1 (ang1) and angiopoietin-2 (ang2) in mouse embryonic development. Findings suggest ang1 and ang2 play roles in the vascularization of the lung and pancreas.
Area of Science:
- Developmental Biology
- Molecular Biology
- Vascular Biology
Background:
- Angiopoietins are key regulators of angiogenesis.
- Understanding embryonic vascular development is crucial for neonatal pulmonary vasculature issues.
Purpose of the Study:
- To analyze blood vessel formation in developing mouse pancreas and lung.
- To investigate the roles of angiopoietin-1 (ang1) and angiopoietin-2 (ang2) as angiogenesis factors.
Main Methods:
- Gene expression analysis using reverse transcriptase-polymerase chain reaction (RT-PCR) and Southern blotting.
- Immunohistochemistry for von Willebrand factor to map vascular development.
Main Results:
- Ang1 and ang2 gene expression was detected in embryonic mouse lungs (E9.5-postnatal day 1) and pancreas (E12.5-E18.5).
- Vascularization in both organs began by embryonic day 12.5 and increased with gestational age.
- Southern blot confirmed ang2 expression in lung and pancreas.
Conclusions:
- Angiopoietin-1 and angiopoietin-2 are implicated in the vascular development mechanisms of the embryonic mouse lung and pancreas.
